Journal: International Journal of Molecular Sciences

Article Title: Protective Effects of Zerumbone on Colonic Tumorigenesis in Enterotoxigenic Bacteroides fragilis (ETBF)-Colonized AOM/DSS BALB/c Mice

doi: 10.3390/ijms21030857

Figure Lengend Snippet: Experimental scheme and effects of zerumbone on body weight and the disease activity index in enterotoxigenic Bacteroides fragilis (ETBF)/AOM/DSS mice. BALB/c mice were given a single intraperitoneal injection of AOM (10 mg/kg) and provided drinking water ad libitum containing clindamycin/gentamicin for 5 days (yellow box). ETBF was orally inoculated and the antibiotic cocktail was continued for an additional 7 days (yellow box). Seven days later, BALB/c mice were subjected to two cycles of 1% DSS (5 days per cycle, orange boxes) and distilled water (DW; 16 days per cycle, blue boxes). During the two DSS cycles, BALB/c mice were given zerumbone (30, 60 mg/kg, p.o., three times a week). The total period of the experiment was 9 weeks. ( A ) Experimental design of the ETBF/AOM/DSS-induced tumorigenesis model. ( B ) Body weight. ( C ) Disease activity index (DAI). Body weight and DAI were measured at the last day of ETBF/AOM/DSS-treated experiments. S, sham control; E, ETBF; A, AOM; D, DSS; Z (30), Zerumbone (30 mg/kg) and Z (60), Zerumbone (60 mg/kg). Each dot represents one mouse ( n = 6–12 mice per group). Scatter plot. Horizontal bar, median. * p < 0.05, *** p < 0.001. ns, no statistical significance.

Article Snippet: Colonization of bacteria in mouse stool was monitored by serial dilution and plating of stool on brain heart infusion agar (BHIA) plates containing 50 μg/mL of gentamicin (Corning, New York, NY, USA) and 6 μg/mL of clindamycin (Hospira, Chicago, IL, USA).

Techniques: Activity Assay, Injection, Control